Comparative study of six alternative solutions for embalming dogs for gross dissection and as fixatives for histological studies (Record no. 7401)

MARC details
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fixed length control field 03128nam a2200277 i 4500
003 - CONTROL NUMBER IDENTIFIER
control field CHED
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250321152725.0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
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008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250312e2018 ph ||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency Commission on Higher Education
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Cauilan, Jojo D.
245 00 - TITLE STATEMENT
Title Comparative study of six alternative solutions for embalming dogs for gross dissection and as fixatives for histological studies
Statement of responsibility, etc. / Jojo D. Cauilan
260 3# - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Los Baños, Laguna
Name of publisher, distributor, etc. : University of the Philippines Los Baños
Date of publication, distribution, etc. ,2018.
300 ## - PHYSICAL DESCRIPTION
Extent xvi, 121 pages
Dimensions 27 x 21 cm.
500 ## - GENERAL NOTE
General note Thesis (Master of Science in Veterinary Medicine) -- University of the Philippines Los Baños,May2018.
520 3# - SUMMARY, ETC.
Summary, etc. This study was conducted to compare the efficiency of six alternative fixative<br/>solutions with formalin fixation of dog specimen for gross and histological studies.<br/>thirty-five non-descript dogs with the body condition score range of 3-5 out of 1-9<br/>were used and euthanized via the common carotid artery. They were divided into seven<br/>treatment groups with five animals per treatment by stratified sampling. Animals in each<br/>treatment group were embalmed with formaldehyde (Tl), Soap Ethanol-based Fixing<br/>Solution (T2), Weigner's Solution (T3), modified SEFS 1 (T4), modified SEFS 2 (T5),<br/>modified Weigner's Solution 1 (T6) and modified Weigner's Solution 2 (T7). Color,<br/>texture, haptic property and conjunctival irritation scoring was done once a month for five<br/>months. <br/><br/>Bacterial and fungal isolation was conducted to determine the presence of<br/>microbes. One non-descript dog was used for histological study and tissue samples were<br/>collected, fixed with the six alternative solutions and formalin solution, then subjected to<br/>routine paraffin technique at 24 hours, 48 hours and 72 hours post-fixation.<br/>Results revealed that dog specimen fixed with solution from Treatment 6 presented<br/>a life-like color and consistencies which are very important in studying gross anatomy.<br/><br/>However, histological studies revealed that alternative fixing solutions were less effective<br/>with formalin fixation. Fungal and bacterial identification revealed the presence of<br/>microbes while no fungal growths were observed. Cost analysis showed that formalin<br/>solution remains the cheapest of the solutions.<br/>In conclusion, Treatment 6 can be used as an alternative fixative for gross<br/>anatomical studies considering the factors in selection of the type of fixative required for<br/>dissection. Histologically, formalin solution was still the best solution for tissue fixation<br/>although alternative fixative solutions presented in the study can be used but only for a<br/>limited time period of24 hours.
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Embalming
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Dogs
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Dissection
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Histology
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Fixatives (Histology)
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Veterinary Medicine
856 4# - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://181.215.242.151/cgi-bin/koha/opac-retrieve-file.pl?id=7bf89fe729b7b5550b5f6bc52f9f0e02">http://181.215.242.151/cgi-bin/koha/opac-retrieve-file.pl?id=7bf89fe729b7b5550b5f6bc52f9f0e02</a>
Public note Abstract
856 4# - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://181.215.242.151//cgi-bin/koha/opac-retrieve-file.pl?id=8497c576d61147a265b4305f478160d6">http://181.215.242.151//cgi-bin/koha/opac-retrieve-file.pl?id=8497c576d61147a265b4305f478160d6</a>
Public note Table of Contents
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Library of Congress Classification
Koha item type CHED Funded Research
Suppress in OPAC No
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Price effective from Koha item type Shelving location
    Library of Congress Classification     Thesis and Dissertation Commission on Higher Education Commission on Higher Education 03/12/2025   LG 995 2018 C6 C38 CHEDFR-000331 03/12/2025 03/12/2025 CHED Funded Research  
    Library of Congress Classification     Digital Thesis and Dissertation Commission on Higher Education Commission on Higher Education 03/12/2025   LG 995 2018 C6 C38 DCHEDFR-000077 03/12/2025 03/12/2025 CHED Funded Research Digital Thesis and Dissertation
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