Molecular identification of genus Conus (Conidae gastropoda) at Verde Island, Batangas, Philippines using mitochondrial gene markers cytochrome oxidase 1 and 16s rRNA (Record no. 7423)

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control field CHED
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250422160030.0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
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008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250411e2018 ph ||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency Commission on Higher Education
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Payawal, Ruthela P.
245 00 - TITLE STATEMENT
Title Molecular identification of genus Conus (Conidae gastropoda) at Verde Island, Batangas, Philippines using mitochondrial gene markers cytochrome oxidase 1 and 16s rRNA
Statement of responsibility, etc. / Ruthela P. Payawal
260 3# - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Nueva Ecija
Name of publisher, distributor, etc. : Central Luzon State University
Date of publication, distribution, etc. ,2018.
300 ## - PHYSICAL DESCRIPTION
Extent xi, 84 leaves
Dimensions ; 27 x 21cm.
500 ## - GENERAL NOTE
General note Thesis (Master of Science in Biology) -- Central Luzon State University, September 2018.
520 3# - SUMMARY, ETC.
Summary, etc. The Verde Island Passage is home to a great diversity of marine organisms. Given its<br/>diversity, this presents an opportunity for discovering economically and pharmacologically<br/>important Conus species of which a record on the available and remaining Conus species in the<br/>area is needed in building data on the regional collection of this group. To gather accurate data,<br/>identification using mitochondrial gene markers l6S rRNA and COI were employed.<br/>Forty-five (45) samples were collected and initially separated by phenotype. DNA was<br/>extracted and the mitochondrial CO1 and 16S gene markers were amplified using Polymerase<br/>Chain Reaction using specific primers. Sequences were assembled and subjected to NCBI BLAST<br/>for identification. Molecular phylogeny was generated using Neighbor Joining, Maximum<br/>Parsimony and Maximum Likelihood Methods.<br/>Twenty-one (21) distinct species were identified for both the gene markers. Phylogenetic<br/>analysis in both gene markers of majority of the samples revealed consistency with the molecular<br/>phylogeny of Conus species. Twenty six (26) samples had matching identities using the two gene<br/>markers, thereby confirming their species identification. Eleven samples (11) had differences in<br/>identification. Tree topologies in both COI and 16S rRNA were essentially similar. Most species<br/>formed well defined lineages and consistent clades with robust statistical supports.
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Conus (Gastropoda)
Geographic subdivision Philippines—Batangas (Province)
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cytochrome oxidase
General subdivision Genetic aspects
650 20 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RNA, Ribosomal, 16S
General subdivision Genetic aspects
856 40 - ELECTRONIC LOCATION AND ACCESS
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Public note Abstract
856 40 - ELECTRONIC LOCATION AND ACCESS
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Public note Table of Contents
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    Library of Congress Classification   Storage Area Commission on Higher Education Commission on Higher Education CHED Funded research 04/11/2025   LG 995 2018 C6 P39 CHEDFR-000343 04/11/2025 04/11/2025 CHED Funded Research    
    Library of Congress Classification     Commission on Higher Education Commission on Higher Education Digital Thesis and Dissertation 04/11/2025   LG 995 2018 C6 P39 DCHEDFR-000090 04/11/2025 04/11/2025 Digital Thesis and Dissertations Room Use Only Digital Thesis and Dissertation
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