Extraction process optimization and evaluation of physico-chemical and functional properties of gelatin from sea slug (Peronia verruculata Cuv.)
/ Windesa Balili Yap
- Leyte : Visayas State University ,2018.
- x, 92 leaves 29 x 20cm.
Thesis (Master of Science in Food Science and Technology) -- Visayas State University, June 2018.
The extraction process of gelatin from sea slug (Peronia verruculata Cuv.) was optimized using Response Surface Methodology (RSM) involving 3-factors, 3-levels Central Composite Design (CCD) which was done after screening of variables using Plackett-Burman (PB) Design. The seven (7) variables that were screened for optimization were particle size, Sodium Hydroxide (NaOH) concentration, type of strong acid used, citric acid concentration, extraction time, extraction temperature and slug to water ratio. These variables were found relevant in sea slug gelatin extraction during preliminary experiment. Screening results suggested that factors that were highly influential for the extraction of gelatin were extraction time, extraction temperature and slug to water ratio. Optimization results showed that the optimum conditions to process the extraction of gelatin from sea slug obtained when extraction temperature is reached at the maximum level of 98°C and a slug to water ratio of 1: 11.3, regardless of the extraction time.