000			03128nam a2200277 i 4500
003			CHED
005			20250321152725.0
007			ta
008			250312e2018 ph ||||| |||| 00| 0 eng d
040			_cCommission on Higher Education
100	1		_aCauilan, Jojo D.
245	0	0	_aComparative study of six alternative solutions for embalming dogs for gross dissection and as fixatives for histological studies _c / Jojo D. Cauilan
260	3		_aLos Baños, Laguna _b : University of the Philippines Los Baños _c,2018.
300			_axvi, 121 pages _c27 x 21 cm.
500			_aThesis (Master of Science in Veterinary Medicine) -- University of the Philippines Los Baños,May2018.
520	3		_aThis study was conducted to compare the efficiency of six alternative fixative solutions with formalin fixation of dog specimen for gross and histological studies. thirty-five non-descript dogs with the body condition score range of 3-5 out of 1-9 were used and euthanized via the common carotid artery. They were divided into seven treatment groups with five animals per treatment by stratified sampling. Animals in each treatment group were embalmed with formaldehyde (Tl), Soap Ethanol-based Fixing Solution (T2), Weigner's Solution (T3), modified SEFS 1 (T4), modified SEFS 2 (T5), modified Weigner's Solution 1 (T6) and modified Weigner's Solution 2 (T7). Color, texture, haptic property and conjunctival irritation scoring was done once a month for five months. Bacterial and fungal isolation was conducted to determine the presence of microbes. One non-descript dog was used for histological study and tissue samples were collected, fixed with the six alternative solutions and formalin solution, then subjected to routine paraffin technique at 24 hours, 48 hours and 72 hours post-fixation. Results revealed that dog specimen fixed with solution from Treatment 6 presented a life-like color and consistencies which are very important in studying gross anatomy. However, histological studies revealed that alternative fixing solutions were less effective with formalin fixation. Fungal and bacterial identification revealed the presence of microbes while no fungal growths were observed. Cost analysis showed that formalin solution remains the cheapest of the solutions. In conclusion, Treatment 6 can be used as an alternative fixative for gross anatomical studies considering the factors in selection of the type of fixative required for dissection. Histologically, formalin solution was still the best solution for tissue fixation although alternative fixative solutions presented in the study can be used but only for a limited time period of24 hours.
650	1	0	_aEmbalming
650	2	0	_aDogs
650	2	0	_aDissection
650	2	0	_aHistology
650	2	0	_aFixatives (Histology)
650	2	0	_aVeterinary Medicine
856	4		_uhttp://181.215.242.151/cgi-bin/koha/opac-retrieve-file.pl?id=7bf89fe729b7b5550b5f6bc52f9f0e02 _zAbstract
856	4		_uhttp://181.215.242.151//cgi-bin/koha/opac-retrieve-file.pl?id=8497c576d61147a265b4305f478160d6 _zTable of Contents
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