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005			20250422160030.0
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008			250411e2018 ph ||||| |||| 00| 0 eng d
040			_cCommission on Higher Education
100	1		_aPayawal, Ruthela P.
245	0	0	_aMolecular identification of genus Conus (Conidae gastropoda) at Verde Island, Batangas, Philippines using mitochondrial gene markers cytochrome oxidase 1 and 16s rRNA _c / Ruthela P. Payawal
260	3		_aNueva Ecija _b : Central Luzon State University _c,2018.
300			_axi, 84 leaves _c ; 27 x 21cm.
500			_aThesis (Master of Science in Biology) -- Central Luzon State University, September 2018.
520	3		_aThe Verde Island Passage is home to a great diversity of marine organisms. Given its diversity, this presents an opportunity for discovering economically and pharmacologically important Conus species of which a record on the available and remaining Conus species in the area is needed in building data on the regional collection of this group. To gather accurate data, identification using mitochondrial gene markers l6S rRNA and COI were employed. Forty-five (45) samples were collected and initially separated by phenotype. DNA was extracted and the mitochondrial CO1 and 16S gene markers were amplified using Polymerase Chain Reaction using specific primers. Sequences were assembled and subjected to NCBI BLAST for identification. Molecular phylogeny was generated using Neighbor Joining, Maximum Parsimony and Maximum Likelihood Methods. Twenty-one (21) distinct species were identified for both the gene markers. Phylogenetic analysis in both gene markers of majority of the samples revealed consistency with the molecular phylogeny of Conus species. Twenty six (26) samples had matching identities using the two gene markers, thereby confirming their species identification. Eleven samples (11) had differences in identification. Tree topologies in both COI and 16S rRNA were essentially similar. Most species formed well defined lineages and consistent clades with robust statistical supports.
650	1	0	_aConus (Gastropoda) _zPhilippines—Batangas (Province)
650	2	0	_aCytochrome oxidase _xGenetic aspects
650	2	0	_aRNA, Ribosomal, 16S _xGenetic aspects
856	4	0	_uhttp://181.215.242.151/cgi-bin/koha/opac-retrieve-file.pl?id=cd4fa8c8ef15a0764351a54856e6fbaf _zAbstract
856	4	0	_uhttp://181.215.242.151/cgi-bin/koha/opac-retrieve-file.pl?id=b4e07496b0839ec1d50c3fa6358f1b53 _zTable of Contents
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