Inhibitory potential of Chitosan against identified spoilage microorganism in moron and shelf-life studies of chitosan-treated moron / Clyde Dwynelh Orillo Garcia

Thesis (Master of Science in Food Science and Technology) -- Visayas State University, June 2018.

Moron, a traditional delicacy in the Philippines, is susceptible to spoilage. One
of the contributing factors of its spoilage is microbial growth. Many researchers have
tried extending the shelf stability of food including the use of chitosan. The use of
chitosan against microbial activity is widely documented. This study aimed to isolate
and identify two of the causative microorganisms that contributes to the deterioration
of Moron and evaluate the efficacy of chitosan as microbial growth inhibitor against
the identified microorganisms using the agar well method.
The isolated microorganisms from Moron were found to be Bacillus cereus and
Fusarium species which are generally dominant in rice-based products and banana wilt
disease, respectively. Results also revealed that chitosan is inhibitory against the
isolated microorganisms. 1% chitosan was found to be more effective against Bacillus
cereusthan the 2% exhibited by the measure of zone of inhibition (10.5mm and 9.5mm,
respectively) while 2% chitosan gave a greater zone measure than 1 % against Fusarium
sp (14mm and 11mm, respectively).